nCounter Analysis System
SOURCES SOUGHT NOTICE TEMPLATE
1. Solicitation Number: HHS-NIH-NIDA-SSSA-SBSS-2014-425
2. Title: nCounter Analysis System
2. Classification Code: 66
3. NAICS Code: 334516
4. Description: nCounter Analysis System NCT-SYST-200
This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.
This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only.
Background: The National Institute on Aging (NIA), one of the 27 Institutes and Centers of NIH, leads a broad scientific effort to understand the nature of aging and to extend the healthy, active years of life. The Intramural Research Program (IRP) of the National Institute on Aging (NIA) provides a stimulating setting for a comprehensive effort to understand aging through multidisciplinary investigator-initiated research, in this regard..
Purpose and Objectives: The nanoString nCounter system represents the state-of-the art technology for multiplexed pathway-based translational research or validation of gene targets identified by next generation sequencing technology. Input samples ranges from single cells, tissue extracts, whole blood lysates, total RNA, cell lysates, archival (paraffin)-embedded tissues, and rare-event cells isolated from laser micro-dissection. There are pre-built panels covering the range of genes involved in inflammation, cancer, immunology from mouse and human samples. Currently, there are 2 systems at NIH (NCI main campus) and one at Johns Hopkins SOM.
This new technology employs propriety 6 reporter molecules linked in a linear manner to gene target, allowing 6 (Factorial) or 720 possible target genes to be simultaneously detected and can provide the following information:
• Multiplexed protein analysis at the single cell level
• Gene expression analysis, up to 800 RNA transcripts with internal controls for quantitation and reproducibility with higher sensitivity than conventional qPCR
• Single cell gene expression analysis, with lower variability that what is seen with analog methods
• IncRNA expression analysis
• miRNA expression and miRGE expression analysis
• Copy number variation analysis
• ChiP-string expression analysis
• User-customizable gene panels or add probes to code sets
• Automated with rapid turnaround of gene analysis
• LMG specific applications
o RecQ5 expression and analysis done under different experimental conditions
o Knockdown of RecQ expression and follow effect on repair pathway over time and/or conditions are placed under stress.
o Evaluate any incestuous relationships between RecQ5 and other DNA repair pathways.
Anticipated period of performance: 30 days after award
Capability statement /information sought. Interested organizations must provide clear and convincing documentation of their capability of providing the items specified in this notice. Interested organizations that believe they possess the ability to provide the required items must submit specific documentation of their ability to meet each of the project requirements to the Contract Specialist. Interested organizations must provide their Company Name, DUNS number, Physical Address, and Point of Contact Information.
Interested organizations are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration.
The Government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number.
The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc.) pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements.
One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2" x 11" paper size, with 1" top, bottom, left and right margins, and with single or double spacing.
The information submitted must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required.
The response is limited to ten (10) page limit. The 10-page limit does not include the cover page, executive summary, or references, if requested.
The response must include the respondents' technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses.
All capability statements sent in response to this Sources Sought Notice must be submitted electronically (via email) to Rodney Brooks, Contract Specialist, at [email protected] in MS Word format by or before the closing date of this announcement. All responses must be received by the specified due date and time in order to be considered. Facsimile responses are NOT accepted.
The response must be received on or before July 14, 2014 12 pm, eastern time.
"Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work.
Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation.
Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
Contracting Office Address:
31 Center Drive
Room 1B59
Bethesda, Maryland 20892
Primary Point of Contact.:
Rodney Brooks
[email protected]
Rodney E. Brooks, Contract Specialist, Phone 3014020751, Email [email protected]
