Targeted knock-in of a gene in the mouse genome
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is NICHD17-124 and is issued as a Request for Quotation (RFQ). The solicitation/contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2005-95. The North American Industrial Classification (NAICS) code 541711 with a size standard of 500. However, this solicitation is not set aside for small business. This acquisition is being conducted using Simplified Acquisition Procedures in accordance with FAR Part 13. The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases intend procure services providing Targeted knock-in of a gene in the mouse genome.
Thyroid hormone is important for promoting development and for maintaining general health in adulthood. Type 3 deiodinase (encoded by Dio3 gene) is a thyroid hormone-inactivating enzyme. It is present in in embryonic, postnatal and adult tissues. Its expression pattern has suggested a protective role of Dio3 in immature and developing organs. To understand more precisely the tissues and cell types in which type 3 deiodinase functions, it is important to be able to trace accurately the expression pattern of the Dio3 gene. This has been a difficult challenge because the protein is expressed at low levels. There is no antibody available that reliably detects type 3 deiodinase in natural tissues. The knock-in mouse model proposed in this project is designed to bypass these difficulties and allow sensitive detection of type 3 deiodinase protein with precise cellular resolution in natural tissues.
The goal of the work is to generate a mouse strain that carries a specific, heritable alteration in one of its genes. The task involves the application of gene targeting technology which is based on the process of homologous recombination to introduce the mutation into the desired gene. This is performed in mouse embryonic stem cells which are used to create founder mice carrying the targeted mutation. The mutation should be transmitted through the germline of the founder mice to allow generation of a colony of mice carrying the mutation for analysis.
The offeror must include a completed copy of the provision of FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items with its offer. The provisions of FAR Clause 52.212-1 Instructions to Offerors - Commercial Items; FAR Clause 52.212-2, Evaluation - Commercial Items; FAR Clause 52.212-4, Contract Terms and Conditions - Commercial Items; and FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items - Deviation for Simplified Acquisitions applies to this acquisition. The offeror must include their Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. The clauses are available in full text at http://www.acquisition.gov/far/. Interested vendors capable of furnishing the government with the product specified in this synopsis should submit a copy of their quotation to the below address in this solicitation. Offers must also be accompanied by descriptive literature, delivery timeframe, warranties and/or other information that demonstrates that the offer meets all of the foregoing requirements. Quotations will be due on July 27, 2017, before 11:00am EST via email or postal mail. The quotation must reference "Solicitation number" NICHD17-124. Quotations sent by postal mail or other mailing services must be submitted to the following address: Eunice Kennedy Shriver National Institute of Child Health and Human Development 6710B Rockledge Drive, Room 1155A, MSC 7000 Bethesda, MD 20892. Note: Fed Ex/Ups/other courier use 20817. Attention: Verne Griffin, by the date and time mentioned above. Any questions must be sent via email to verne.griffin@nih.gov and must include solicitation# NICHD2017025 in the subject line of email.
Faxed copies/responses will not be accepted. Note: In order to receive an award, contractor must be registered and have valid certification for all awards in the SAM database @www.sam.gov.
Verne Griffin, Email verne.griffin@nih.gov
