SEQUENCING SERVICES
The National Institutes of Health, National Institute Of Allergy and Infectious Diseases (NIH/NIAID) has a sole source requirement and intends to negotiate for the sequencing of T and B cell receptors using Sanger technology for individual PCR reactions. from Beckman Coulter, Inc. , 250 S. Kraemer Blvd., Brea, CA 92821-6232 on an other than full and open competition to supply all the necessary reagents and provide the instrumentation to acquire NIH samples and sequence as listed in the Contract Requirements below as also shown on the Statement of Work attached.
Contract Requirements
The proposed contract will guarantee the following:
· PCR amplicon purification using AMPure
· Dye Terminator fluorescent sequencing reaction set up
· Sequence product purification using CleanSEQ
· Detection of sequenced fragments by capillary electrophoresis
· Generation of results by Chromatograms, PHRED wuality scores and FASTA sequence files.
· All results will be in a timely manner between 24-48 hours or as short a time frame as possible depending on volume of samples submitted.
All responsible sources may submit a proposal showing their capabilities and past performance. All responsible sources may submit a response that will be considered. The Government reserves the right to make a decision whether to this requirement is available for competition. All responsible sources may submit a response that will be considered. The Government reserves the right to make a decision whether to this requirement is available for competition. All responses shall be submitted no later than 4:00 p.m. (EDST), June 24, 2014. For delivery through the U.S. Postal Service, the address is NIH/NIAID/AMOB, 10401 Fernwood Road, 2NE52E, Bethesda, MD, 20817-4811. Electronic and Fax copies are not accepted.
Merle J. Dixon, Purchasing Agent, Phone 3014026162, Fax 3014022285, Email [email protected] - Sylvia Kate - Robinson, Contracting Officer, Phone 301-496-2607, Fax 301-402-2285, Email [email protected]
